A no-trust motion was tabled in the Balochistan Assembly against Speaker Aslam Bhotani on Wednesday.
Voting on the resolution will be held today (Thursday).
Reacting to the resolution, Bhotani said he would make no compromise on his principled stand to save his job.
The PPP-led alliance held consultations to move a no-trust motion against Bhotani. Eleven lawmakers reportedly opposed the plan.
Balochistan Assembly Deputy Speaker Matiullah presided over the session, in which Health Minister Ainullah Shams eventually presented the resolution.
Speaking to a private television channel, Bhotani said he would not change his stand to save his position. He said he would continue to work for the supremacy of constitution.
Speaking to reporters, Chief Minister Aslam Raisani said independent candidates were not backing the move.